Research Scientist/Engineer Assistant

Job Description

RESEARCH SCIENTIST/ENGINEER ASSISTANT

Req #: 237253 Department: BIOCHEMISTRY Job Location: Seattle - South Lake Union Posting Date: 07/29/2024 Closing Info: Open Until Filled Salary: $3,629 - $4,129 per month Other Compensation: Union Position: Yes Shift: First Shift Benefits: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills, and dedication to build stronger minds and a healthier world.
UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits, and natural beauty.
The Department of Biochemistry has an outstanding opportunity for a Research Scientist/Engineer Assistant to join their team.
POSITION PURPOSE
This research project will aim to determine how immunological memory develops and how memory cells inherit their cellular identity beyond initial antigen presentation and immune system activation.
DUTIES AND RESPONSIBILITIES
• Develop and perform laboratory procedures in the execution of specific research projects with human induced pluripotent stem cells (hiPSCs) and mouse stem cells – 40%
• Help with Biochemistry techniques such as protein purifications and in vitro characterization of proteins – 20%
• Work with senior scientists including Principle Investigator to Collect, analyze, and maintain records of research data – 15%
• Lab operations: Maintaining lab supplies, organizing lab records, and reagents for research projects – 15%
• Help with grant writing and publication of research results – 10%
MINIMUM REQUIREMENTS
Bachelor's Degree in Biochemistry and less than one year of relevant experience, OR Equivalent combination of education/experience.
Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.
ADDITIONAL REQUIREMENTS
• Understanding of Biochemistry methods with in vitro enzymatic assay experience.
• Ability to perform molecular biology techniques such as DNA isolation, PCR, qPCR, cloning, electrophoresis, western blot, and immunoprecipitations.
• Ability to perform tissue culture techniques for human induced pluripotent stem cells (hiPSCs) and maintain differentiation cultures.
• Ability to perform lenti/retrovirus production and transduction protocols.
• Ability to maintain a sterile environment.
• Experience creating reagants and aliquots.
• Evidence of ability to analyze, present, and write data clearly.
• Strong organizational and communication skills and ability to work as a member of a team.
DESIRED QUALIFICATIONS
• Ability to work with mouse primary cells.
• Experience genotyping mice.
• Ability to extract DNA from mouse tissue samples.
• Knowledge with flow cytometry and sorting techniques of mouse and human stem cells.
• Knowledge with immunostaining and quantitative morphometric analysis of cell phenotypes.
• Experience with microscopy.
• Knowledge of protein purification techniques.
• Experience working with human stem cells.
• Knowledge and understanding of CRISPR techniques.
• Knowledge of Cas9-gRNA transfection techniques in mice.
• Experience cleaning and maintaining lab equipment.
Application Process: The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

Committed to attracting and retaining a diverse staff, the University of Washington will honor your experiences, perspectives and unique identity. Together, our community strives to create and maintain working and learning environments that are inclusive, equitable and welcoming.

The University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, gender expression, national origin, age, protected veteran or disabled status, or genetic information.

To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or [email protected] .

Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law .

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