Research Scientist/Engineer 3

Job Description

RESEARCH SCIENTIST/ENGINEER 3

Req #: 236714 Department: DEPARTMENT OF LABORATORY MEDICINE AND PATHOLOGY Job Location: Seattle Campus Posting Date: 07/31/2024 Closing Info: Open Until Filled Salary: $5,427- $9,000 per month Other Compensation: Union Position: Yes Shift: First Shift Benefits: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills and dedication to build stronger minds and a healthier world.
UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty.
The Department of Laboratory Medicine and Pathology has an outstanding opportunity for a Research Scientist/Engineer 3 to join their team.
The 3D structure of eukaryotic genomes plays a central role in gene regulation, and aberrant structures are now recognized to affect a wide range of diseases. However, few approaches have been developed to correct aberrant chromatin loops and positioning within the nucleus. We seek to develop methods to efficiently engineer chromatin loops and recruit long non-coding RNAs (lncRNAs) to move specific chromatin regions within the nucleus, using CRISPR-Cas systems that target either genomic DNA or RNA. These methods will be used to regulate expression of disease-associated genes on the X chromosome, which will enable fundamental studies on the role of spatial organization in the control of gene expression and X chromosome inactivation. It will also provide new approaches to treat diseases that arise from mutations in X-linked genes, enabling precise restoration of native expression levels in dosage-sensitive genes. The proposed approaches will utilize the X chromosome as a model system, but will also be applicable to genes in other regions of the genome.
DUTIES AND RESPONSIBILITIES
This position will support research activities including but not limited to:
• 25% Construction of CRISPR-Cas9/Cas13 derived vectors.
• 25% Microscopy analysis to look for chromatin loop formation and repositioning.
• 20% Culture of mouse and human cells.
• 15% Measurements of gene expression and of chromatin structure.
• 10% Contribution to data collection, report writing, and presentation of results.
• 5% Participation in training of laboratory staff.
MINIMUM REQUIREMENTS
• Bachelor's degree in Chemistry, Biochemistry, Genetics, or Molecular Biology AND 4 years of related work experience OR Equivalent combination of education/experience
Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.
Application Process:
The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

Committed to attracting and retaining a diverse staff, the University of Washington will honor your experiences, perspectives and unique identity. Together, our community strives to create and maintain working and learning environments that are inclusive, equitable and welcoming.

The University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, gender expression, national origin, age, protected veteran or disabled status, or genetic information.

To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or [email protected] .

Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law .

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